Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant EBFP (NCBI accession number AX_766758 region: 1-717, expression vector pGEX-1N), expressed in E. coli.
Form
Supplied as a lyophilized powder in 10mM ammonium bicarbonate, BSA. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes recombinant EBFP.
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