Blue alpha Gal CAS

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B2175-05 Blue alpha Gal CAS:

Specifications

References

Grade

Molecular Biology Grade

Molecular Formula

C14H16BrNO6

Molecular Weight

374.2

EU Commodity Code

38220090

Shipping Temp

Storage Temp

-20°C

5-Bromo-3-Indolyl-a-D-galactoside (galactopyranoside)

Blue Alpha Gal is a chromogenic substrate for alpha-galactosidase.

Storage and Stability

Store powder at -20°C under nitrogen. Powder is stable for 12 months at -20°C. Aliqout and store stock solutions of X-a-Gal at -20°C. Aliquots are stable for at least 6 months at -20°C. Protect from light and humidity.

Purity

≥ 98% (HPLC)

Appearance

White to off white powder.

Solubility

1% in DMF is clear and practically colorless

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

1. S. Fields and O.-K. Song, A Novel Genetic System to Detect Protein-Protein Interactions. Nature 340, 245-24 (1989). 2. S. Aho, A. Arffman, T. Pummi and J. Uitto, A novel reporter gene MEL1 for the yeast two-hybrid system. Anal. Biochem. 253, 270-272(1997). 3. R.S. Tubb and P.L. Liljestrom, A colony-colour method which differentiates a-galactosidase positive strains of yeast. J. Inst. Brew. 92, 588, (1986). 4. R. Gossrau and Z. Lojda, Histochemical detection of -D-galactosidase with 5-Br-4-Cl-3-indoxyl a-D-galactoside. Acta histochem. 85, 213 (1989). 5. P. Chevalier, D. Roy and and L. Savoie, X- a-gal-based medium for simultaneous enumeration of bifidobacteria and lactic acid bacteria in milk. J. Microbiol. Meth. 13, 75 (1991). 6. American Society of Brewing Chemists. Report of Subcommittee on X- a-gal medium. J. Amer. Soc. Brew. Chem. 51, 185, (1993).