Bone morphogenetic protein 10 (BMP-10) is a TGF-b superfamily member whose expression is restricted to the developing and postnatal heart. It is synthesized as a 424aa pre-proprotein with a putative 21aa signal peptide, a 292aa propeptide, and a 108aa mature protein. Mature BMP-10 is secreted as a 25kD disulfide-linked homodimer. The mouse BMP-10 proprotein shares 86-95aa sequence identity with human and rat BMP-10, respectively.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa314-421 from human BMP-10 expressed in CHO cells (Q9R229).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes mouse BMP-10. Species sequence homology: recombinant human BMP-10, rhBMP-8a (10%), recombinant mouse BMP-8b. Does not react with rmBMP-3, -4, -5, -6, -7, -9, -15, rhBMP-1.1, -2, -3b, propeptide rhBMP-10.
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