Brilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for typhoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive and most Gram negative bacteria. Phenol red serves as a pH indicator for the fermentation of lactose or sucrose in the medium.

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Light beige with a greenish tint, homogeneous, free flowing powder.

Brownish-green, slightly turbid.

6.9±0.2

Escherichia coli ATCC 25922: Inhibited Salmonella cholerasius ATCC 13076: Growth, Pink colonies Salmonella typhi ATCC 19430: Inhibited Salmonella typhimurium ATCC 14028: Growth, Pink colonies Staphylococcus aureus ATCC 25923: Inhibited

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.