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C0075-03D Mouse Anti-Sialyl Lewis A (CA19-9 Cancer Antigen)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
121SLE
Grade
Highly Purified
Applications
E IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Sialyl Lewis (a)

Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Paraffin): 10ug/ml using heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour/RT; detection DAB. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.
Form
Supplied as a liquid in TBS, pH 8.0, 15mM sodium azide.
Purity
Purified precipitation methods and size-exclusion chromatography.
Specificity
Recognizes human CA19-9 cancer antigen.
References
1. Rouger Ph. et al. (Eds.): Proceedings of the First International Workshop on monoclonal antibodies against human red blood cells and related antigens (Paris, 1987) published in: Blood Transfusion and Immunohaematology 30 (5), pp 353-720.|2. Rye PD, Bovin NV, Vlasova EV, Molodyk AA, Baryshnikov A, Kreutz FT, Garinther WI, Schultes BC, Noujaim AA, Madiyalakan R, Magnani J, Nilsson O, Nilsson K, Nustad K, Norum L, Bell H, Cao Y, Suresh MR, Very DL, Freeman JV, Yeung KK, Hilgers J: Summary report on the ISOBM TD-6 workshop: analysis of 20 monoclonal antibodies against Sialyl Lewisa and related antigens. Montreux, Switzerland, September 19-24, 1997. Tumour Biol. 1998;19(5):390-420.
USBio References
No references available
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