Calponin is a thin filament associated protein that binds calmodulin, actin and tropomyosin. The multi-gene family of calponins includes calponin 1 (CNN1), 2 (CNN2) and 3 (CNN3). CNN1 is expressed in smooth muscle and tissues containing significant amounts of smooth muscle. This antibody is predicted to detect all three forms of calponin.
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.25-0.5ug/ml Immunohistochemistry (formalin and paraffin): 0.5-1.0ug/ml Optimal dilution determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Turkey gizzard calponin.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.02% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Purity
Purified by affinity chromatography.
Specificity
Recognizes calponin. Species Crossreactivity: human, mouse, rat, and chicken.
TDS
NCBI
UniProt
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