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C1145-45E 5(6)-Carboxyfluorescein Diisobutyrate (CFDI) CAS: 287111-44-8

Specifications
References
CAS Number
287111-44-8
Grade
Highly Purified
Molecular Formula
C29H24O9
Molecular Weight
516.5
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
-20°C
CFDI; 3’,6’-Bis(2-methyl-1-oxopropoxy)-3-oxosSpiro[isobenzofuran-1(3H),9’-[9H]xanthene]-ar-carboxylic Acid

A protected 5(6)-Carboxyfluorescein in which the hydroxyl groups are acylated with isobutyric acid. The protection prevents self-condensation during carboxyl activation andavoids the formation of fluorophore-oligomer containg by-products associated with the use of unprotected FAM.

Synonyms
CFDI; 3’,6’-Bis(2-methyl-1-oxopropoxy)-3-oxosSpiro[isobenzofuran-1(3H),9’-[9H]xanthene]-ar-carboxylic Acid
CAS Number
287111-44-8
Molecular Formula
C29H24O9
Molecular Weight
516.5
Appearance
Supplied as a yellow solid
Purity
≥98%
Melting Point
>138ºC (dec.)
Solubility
Chloroform (Slightly), Methanol (Slightly)
Method for Determining Identity
Proton NMR Spectroscopic and Mass Spectrometric Analysis
TLC Conditions
SiO2; Dichloromethane: Methanol; 9:1; Visualized with UV and AMCS; Rf=0.35
Ethly Acetate Content
~2%
Atmoshere
Inert Gas
Storage and Stability
May be stored at RT for short-term only. Long-term storage is recommended at -20°C for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Store Under inert atmosphere.
References
1. Van der Poel, J.J., et al.: Immunol. Lett., 2:187 (1981), 2. Fries, J.R., et al.: Chimica Oggi, 19:18 (2001), 3. Maly, D.J., et al.: Chembiochem., 3:16 (2002)
USBio References
No references available
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