This ELISA Kit is a solid phase sandwich enzyme-linked im munosorbent assay (ELISA) that detects endogenous levels of total beta-catenin protein (phosphorylated and non-phosphorylated). A beta-Catenin antibody has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-beta-catenin proteins are captured by the coated antibody antibody. Following extensive washing, beta-Catenin mouse monoclonal antibody is added to detect both the captured phospho- and nonphospho-beta-catenin protein. An anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color color. The magnitude of optical density for this developed color is proportional to the quantity of total beta-catenin protein.

1. Microtiter Plate, Catenin antibody-coated microtiter, 1x96 wells. 2. Beta-Catenin Detection Ab, 1x11ml 3. IgG (HRP) Ab, 1x11ml 4. TMB Substrate, 1x 11ml 5. STOP Solution, 1x11ml 6. Wash Buffer (20X), 1x25ml 7. Sample Diluent, 1x25ml. 8. Cell Lysis Buffer, 1x15ml

Store all kit components at 4°C except Cell Lysis Buffer. Store Cell Lysis Buffer at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.