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C2098-69B Rabbit Anti-Cell division cycle associated 8 (BOR, Borealin, BOREALIN, Cell division cycle-associated protein 8, Dasra-B, FLJ10468, FLJ12042, hDasra-B, PESCRG3, Pluripotent embryonic stem cell-related gene 3 protein)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Accession #
NP_060571
Shipping Temp
Blue Ice
Storage Temp
-20°C

CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control: Rat Kidney Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 19aa near the amino terminus of the human CDCA8.
Form
Supplied as a liquid in PBS containing 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CDCA8. Species Crossreactivity: Mouse and rat.
References
1. Gassmann R, Carvalho A, Hanzing AJ, et al. Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. J. Cell Biol. 2004; 166:179-91. 2. Tani M, Okino N, Mitsutake S, et al. Molecular Cloning of the Full-length cDNA Encoding Mouse Neutral Ceramidase. J. Biol. Chem. 2000; 275:11229-34. 3. Hanson KK, Kelley AC, and Bienz M Loss of Drosophila borealin causes polyploidy, delayed apoptosis and abnormal tissue development. Development 2005; 132:4777–87. 4. Date DA, Jacob CJ, Bekier ME et al. Borealin is repressed in response to p53/Rb signaling. Cell Biol. Int. 2007; 31:1470-81.
USBio References
No references available
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