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C2411-22C Mouse Anti-CD57

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
NK-1
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

CD57 is a 110kD antigen present on the peripheral blood lymphocyte subset (primarily large granular lymphocytes). Also found on cells in neuroectodermal tissue.

Applications
Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Frozen): 1:5 Should be fixed with acetone for 5 minutes at -20°C. Immunohistochemistry (Paraffin): 1:5 Should be fixed with buffered formaldehyde without pretreatment. Optimal dilutions to be determined by the researcher.
Positive Control
Tonsil
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Membrane extract of the human lymphoblastoid cell line HSB-2
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity
Purified
Specificity
Recognizes human CD57.
References
1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.|3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD57 Section, Academic Press, New York, p. 287.|4. Abo, T., and C.M. Balch. 1981. J. Immunol. 127:1024.|5. Abo, T., M.D. Cooper, and C.M. Balch. 1982. J. Immunol. 129:1752.|6. d’Angeac, A.D., S. Monier, D. Pilling, A. Travaglio-Encinoza, T. Reme, and M. Salmon. 1994. Eur. J. Immunol. 24:1503.
USBio References
No references available
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