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C2423-05F Rat Anti-CD68 (MACROSIALIN, Macrophage antigen, SCARD1, Scavenger receptor class D member1) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
P31996
Conjugate
FITC
Isotype
IgG2a
Clone Number
6A326 (FA-11)
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homolog of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (LAMP) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required for this application. Optimal dilution determined by the researcher
Recommended Negative Control
I1904-82E1: IgG2a, Rat Negative Control (FITC)
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified Concanavalin A acceptor glycoprotein from P815 cell line
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.|Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes mouse CD68.
References
1. Ramprasad, M.P., et al., Proc. Natl. Acad. Sci. 93: 14,833-14,838 (1996). 2. Rabinowitz, S.S. ety al J. Exp. Med. 174: 827-836 (1991). 3. Da Silva, R.P. and Gordon, S., Biochem. J. 338: 687-694 (1999). 4. Schleicher, U. et al. (2005) Blood 105: 1319-1328. 5. von Lukowicz, T. et al. (2008) Cardiovasc. Res. 78: 158-166 6. Kassim, S. et al. (2010) PloS ONE 5: e13424. 7. Rahaman, S.O. et al. (2011) J Biol Chem. Jan 5. [Epub ahead of print]8. Frossard, J.L. et al. (2011) J Clin Pathol. Feb 23. [Epub ahead of print]9. West, E.L. et al. (2010) Stem Cells. 28: 1997-2007.10. Lopez, M.E. et al. (2011) J Neurosci. 31: 4367-78.11. Jayagopal, A. et al. (2009)Nanotechnology. 20: 165102.12. Leung, V.W. et al. (2009) Am J Pathol. 175: 1757-67.13. Devey, L. et al. (2009) Mol Ther. 17: 65-72.14. Lu, W. et al. (2010) Biomaterials. 31: 2617-26.15. de Beer, M.C. et al. (2003) J Lipid Res. 44: 674-85.16. Song, L. et al. (2011) J Lipid Res. 52: 1542-50.17. Daldrup-Link, H.E. et al. (2011) Clin Cancer Res. Jul 26. [Epub ahead of print]18. Macauley, S.L. et al. (2011) J. Neurosci 31: 15575-8519. Martin-Manso, G. et al. (2008) Cancer Res. 68: 7090-9.20. Lazarini, F. et al. (2012) J Neurosci 32: 3652-64.21. Hemmi, H. et al. (2009) J Immunol. 182: 1278-86.
USBio References
No references available
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