CD235a

Clone Type

Monoclonal

Host

Rat

Source

Human

Swiss Prot

P02724

Isotype

IgG2b

Clone Number

YTH89.1

Grade

Affinity Purified

Applications

FC IHC

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD235a (Glycophorin A (GpA)) antigen is exclusively expressed on human erythroid cells and their progenitors. These antibodies may be effectively used for differentiation in acute leukemias e.g. presence of the CD235a antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells.

Applications

Suitable for use in Flow Cytometry, Immunohistochemistry, and Cytotoxic Assays. Other applications not tested.

Recommended Dilution

Flow Cytometry: 1:10-1:25 Immunohistochemistry (Frozen, paraffin): 1:100-1:1000 . Does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher.

IHC Positive Control

Human hypophysis

Hybridoma

Spleen cells from immunized DA rats were fused with cells of the Y3/Ag.1.2.3.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Human CD235a.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes human CD235a (Glycophorin A (GpA)).

References

1. Outram, S. et al. (1988). Erythromyeloid lineage fidelity is conserved in erythroleukaemia. Leuk. Res. 12: 651-7. 2. Jokiranta, T.S. and Meri, S. (1993) Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement. J. Immunol. 15: 2124-31. 3. Basu, S. (2010) Erythrocyte membrane defects and asymmetry in paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome. Hematology. 15: 236-9.

USBio References

No references available

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C2548-98D Rat Anti-CD235a (Glycophorin A, GpA)

Clone Type

Host

Source

Swiss Prot

Isotype

Clone Number

Grade

Applications

Crossreactivity

Shipping Temp

Storage Temp

References

USBio References

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