Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 1:50. 80ul labels 10x10e6 human red blood cells. Optimal dilutions to be determined by the researcher.

1. Wash 10x10e6 human red blood cells and preincubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. Incubated the cells for 45 minutes on ice with 80ul of C2548-98D3 at a 1:50 dilution. 3. Wash cells three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD235a (Glycophorin A) antibody (C2548-98D1).

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Neuraminidase treated human erythrocytes.

Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).

Purified (90% SDS-PAGE).

Recognizes an extracellular (peptide) domain of human CD235a.