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C2548-98D4 Mouse Anti-CD235a (Glycophorin-A, GPA, GYPA, GPErik, GpMiIII, GPSAT, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN Sialoglycoprotein, MNS, PAS-2, Sialoglycoprotein alpha)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
7H140 (A63-B/C2)
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Glycophorin A (MNS Blood Group)

Human CD235a (glycophorin A) is a sialoglycoprotein expressed early on the cell surface during erythropoiesis as pluripotent stem cells are recruited to differentiate into erythrocytes (1). CD235a (Glycophorin A) is a useful marker to study the developmental biology of hematopoietic progenitor cells (2,3).

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Neuraminidase treated human erythrocytes.
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD235. Reacts with an extracellular (peptide) domain of CD235a (glycophorin A).
References
1. J.G. Mark, et al, (1999) Blood 93: 4425-4435, 2. N. Debili, et al, (1996) Blood 88: 1284-1296, 3. B.M. Frey, et al, (1998) Blood 91: 2781-2792.
USBio References
No references available
Conjugates
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