Cyclin-dependent Kinase 5

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Cyclin-dependent kinase 5 (Cdk5) belongs to the Ser/Thr protein kinase family. It is ubiquitously expressed in both cycling and noncycling cells but its activity is detected almost exclusively in brain extracts. Cdk5 is activated by p35 protein which is only expressed in the brain. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:25 Western Blot: 1:100 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Synthetic peptide (KCNPVQRISAEEALQH) corresponding to aa268-283 of human Cdk5

Form

Supplied as a liquid in PBS, pH 7.2.

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes human Cdk5

References

Pines, J. (1995) Biochem. J. 308,697-711. Maccioni, R. et al. (2001) Eur. J. Biochem. 268,1518-1527. O'Hare, M.J. et al. (2005) J. Neurosci. 25,8954-8966. Angelo, M. et al. (2006) J. Neurochem. 99, 353-370.