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C5069-95 Chromatin Immunoprecipitation (ChIP) Assay Kit, BioAssay™

Specifications
References
Brand
BioAssay™
Kit Type
Assay
Tests
22
Detection Method
Colorimetric/Fluorescent
Sample Matrix
Mammalian cells
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
4°C

For immunoprecipitation of transcriptionally active chromatin from mammalian cells. Detection of gene or promoter of interest interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR or Southern slot-blot analysis, using promotor-specific primer or probe, is recommended

Quantity
22 Chromatin Immunoprecipitation (ChIP) assays.
Kit Components
C5069-95A: Salmon Sperm DNA/Protein A Agarose, 1x1.5ml C5069-95B: ChIP Dilution Buffer, 2x24ml C5069-95C: Low Salt Immune Complex Wash Buffer, 1x24ml C5069-95D: High Salt Immune Complex Wash Buffer, 1x24ml C5069-95E: LiCl Immune Complex Wash Buffer, 1x24ml C5069-95F: TE Buffer, 2x24ml C5069-95G: EDTA, 1x250ul C5069-95H: NaCl, 1x500ul C5069-95J: Tris-HCl, 1x500ul C5069-95K: SDS Lysis Buffer, 1x10ml
Storage and Stability
May be stored at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
References
General References: 1. Luo, R.X., et al., Cell 92: 463-473 (1998). 2. Braunstein, M., et al., Mol. Cell. Biol. 16: 4349-4356 (1996). Application References: 3. Manabe, I., et al., J. Clin. Invest. 107: 823-834 (2001). 4. Cervoni, N. & Szyf, M., J. Biol. Chem. 276: 40,778-40,787 (2001). 5. Gummow, B.M., et al., J. Biol. Chem. 278: 26,572-26,579 (2003). 6. Detich, N., et al., J. Biol. Chem. 278: 27,586-27,592 (2003). 7. Sundaraj, K.P., et al., J. Biol. Chem. 279: 6152-6162 (2004).
USBio References
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