CMRL1066 w/o L-Glutamine, Glucose

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.

Appearance

Orange, homogenous, free flowing powder

Solubility

Orange to yellow, clear, complete

As Reported

Endotoxin

≤1EU/ml

Directions per Liter

Dissolve 8.73g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Chloride, anhy	0.2
Magnesium Sulfate, anhy	0.09769
Potassium Chloride	0.4
Sodium Acetate, anhy	0.05
Sodium Chloride	6.8
Sodium Phosphate Monobasic, anhy	0.122

Amino Acids

L-Alanine	0.025
L-Arginine	0.05787
L-Aspartic Acid	0.03
L-Cysteine•HCL•H2O	0.26
L-Cystine	0.02
L-Glutamic Acid, Sodium	0.0862
L-Glutamine	Absent
Glycine	0.05
L-Histidine•HCl•H2O	0.02
trans-4-Hydroxy-L-Proline	0.01
L-Isoleucine	0.02
L-Leucine	0.06
L-Lysine•HCl	0.07
L-Methionine	0.015
L-Phenylalanine	0.025
L-Proline	0.04
L-Serine	0.025
L-Threonine	0.03
L-Tryptophan	0.01
L-Tyrosine	0.04
L-Valine	0.025

Vitamins

PABA	0.00005
L-Ascorbic Acid•Na	0.05
D-Biotin	0.00001
Choline Chloride	0.0005
Cocarboxylase	0.001
Coenzyme A•Na	0.0025
2'-Deoxyadenosine	0.01
2'-Deoxycytidine•HCl	0.0116
2'-Deoxyguanosine	0.01
Folic Acid	0.00001
myo-Inositol	0.00005
5-Methyldeoxycytidine	0.0001
ß-NAD	0.007
ß-NADP•Na	0.001
Nicotinic Acid	0.000025
Niacinamide	0.000025
D-PantothenicAcid•Ca	0.00001
Pyridoxal•HCl	0.000025
Pyridoxine•HCl	0.000025
Riboflavin	0.00001
Flavin Adenine Dinucleotide•2Na	0.000106
Thiamine•HCl	0.00001

Other

Cholesterol	0.0002
Thymidine	0.01
D-Glucose	Absent
D-GlucuronicAcid•Na	0.00388
Glutathione	0.01
Phenol Red ,Sodium	0.02124
Tween 80	0.005
Uridine-5-Triphosphate•4Na	0.00113

References

1. Healy, G.M., Parker, R.C.: An Improved Chemically Defined Basal Medium (CMRL-1415) For Newly Explanted Mouse Embryo Cells. Journal of Cell Biology 30: 531-538 (1966). 2.Parker, R.C., et al.: Altered Cell Strains In Continuous Culture: A General Survey In: Special Publications of the New York Academy of Sciences 5: 303-313 (1957). Publisher: N.Y. Acad. of Sci.. Ed.: Whitelock, O. 3. Methods of Tissue Culture, Third Edition, pp. 62-80. Publisher: Harper and Row Publishers Inc., New York. Ed.: Parker, R.C.

USBio References

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- Coupon Code

C5900-02A CMRL1066 w/o L-Glutamine, Glucose (Powder)

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