Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.

Heterologous type II collagen is widely used for inducing arthritis in susceptible animals. The serum antibodies in collagen-induced arthritic (CIA) mice are highly specific to the type II collagen used for immunization, however there is significant crossreactivity with other species of type II collagen including rat type II collagen.

This ELISA kit contains enough material to run two partial plates on two separate occasions (see assay procedure).

C7510-34A1: Standard Antibody, 1x20units. Supplied as lyophilized powder. C7510-34A2: IgG (HRP), 2x1vial. Peroxidase-conjugated goat anti-rat IgG. Supplied as lyophilized powder. C7510-34A3: Blocking Buffer, 1x12ml C7510-34A4: Sample/Standard Dilution Buffer, 1x50ml C7510-34A5: IgG (HRP) Dilution Buffer, 1x20ml C7510-34A6: Stop Solution, 1x10ml. 2N sulfuric acid. C7510-34A7: Wash Buffer, 20X, 1x50ml C7510-34A8: OPD Dilution Buffer, 1x20ml C7510-34A9: OPD, 2x1vial C7510-34A10: Microtiter Strips, 1x96 wells. Coated with chicken collagen type II. C7510-34A11: Reference Standard Strips, 4x8-wells. Use 2 strips per run.

Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.