Eller et al. first described Columbia Broth. It was designed to produce rapid and luxuriant growth of a range of organisms due to its high nutrient content. It contains meat peptone, casein peptone and yeast extract all of which are highly nutritious components. The meat peptone contains sufficient sulfonamide antagonists to prevent sulfonamide inhibition of growth. Columbia Broth, in the presence of CO2 and supplemented with SPS, is an excellent blood culture medium.

Bacteroides fragilis ATCC 25285: Good Escherichia coli ATCC 25922: Good Neisseria meningitides ATCC 13090: Good Pseudomonas aeruginosa ATCC 27853: Good Streptococcus pneumonia ATCC 6305: Good Streptococcus pyogenes ATCC 19615: Good

Beige, homogeneous, free flowing powder.

Gold to amber, brilliant to clear, with trace to moderate precipitate

7.3±0.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Dissolve 35g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers, loosen caps and autoclave (15psi) for 15 minutes at 121ºC.

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.