A selective medium for isolation of gram positive bacteria. Most often utilized with blood added. Eliner, et al. added colistin and nalidixic acid to Columbia Agar to suppress the growth of Gram negative bacteria. The addition of 5-10% sheep red blood cells to the base demonstrates hemolytic patterns of the Staphylococci and Streptococci.
Appearance
Light beige, homogeneous, free flowing powder
Solubility
Yellow, slightly opaque after autoclaving
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 43 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Prepare 5-10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C. Dispense into Petri dishes or tubes
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
TDS
NCBI
UniProt
Tech Support
Customer Service
