Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody can be used at a concentration of 10ug/ml to detect human CSL on HASMC (human aortic smooth muscle) cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by NorthernLightsTM 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 5 ng/well. Optimal dilutions should be determined by each laboratory for each application.

Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

E. coli-derived recombinant protein corresponding to Gly208-Ser500 from human CSL.

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

Purified by immunoaffinity chromatography.

Selected for its ability to recognize human CSL in the applications listed.