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D1101-01C Sheep Anti-DARC (Duffy Antigen/Chemokine Receptor, Fy Glycoprotein, Glycoprotein D, Plasmodium Vivax Receptor)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Mo Rt
Accession #
NP_034175
Shipping Temp
Blue Ice
Storage Temp
-20°C

DARC (Duffy Antigen Receptor for Chemokines; also CD234) is a 40-46kD glycoprotein member of the Duffy family of silent heptahelical chemokine receptors. It is expressed in liver and on select neurons, erythrocytes and the endothelium of postcapillary venules. Unlike traditional chemokine receptors, DARC cannot signal through G-proteins as it lacks a DRYLAIVHA cytoplasmic motif. DARC has three potential functions: first, it binds circulating inflammatory-type chemokines, serving as a repository for future chemokine release; second, it acts as a vehicle by which chemokines are transported from the abluminal to the luminal side of endothelium; and third, it complexes with signal-transducing chemokine receptors to create a nonsignaling heterodimer. Mouse DARC is 334aa in length. It contains a 62aa N-terminal extracellular region, and a 28aa C-terminal cytoplasmic tail. There is one potential splice variant that shows a 42aa substitution for aa133-334. Collectively, over the four extracellular domains (aa1-62, 115-127, 186-205, 264-285), mouse DARC shares 52-75aa identity with human and rat DARC, respectively.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.25ug per 10e6 cells Immunohistochemistry: 5-15ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-expressed recombinant protein corresponding to mouse DARC protein fragments (Met1-Pro61; Ala115-Cys127, Ty264-Asn285).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse DARC. Species Crossreactivity: rat.
Conjugates
Pricing
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