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D1876-48P6 Rat Anti-Dectin-1 (C-type Lectin Domain Family 7 Member A, Dendritic Cell-associated C-type Lectin 1, DC-associated C-type Lectin 1, Beta-glucan Receptor, beta-GR, C-type Lectin Superfamily Member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
Q6QLQ4
Conjugate
PE
Isotype
IgG2b
Clone Number
10B2689 (2A11)
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leukocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized Fischer rats were fused with cells of the rat Y3 myeloma cell line
Recommended Negative Control
I1904-84L1: IgG2b Negative Control (PE)
Storage and Stability
Lyophilized powder may be stored at 4°C. Reconstitute with sterile ddH2O. Aliquot and store at 4°C. Reconstituted product is stable for 6 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Immunogen
Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor.
References
1. Brown, G.D., et al. J. Exp. Med. 196: 407-412 (2002). 2. Taylor, P.R., et al., J. Immunol. 169: 3876-3882 (2002). 3. Reid, D.M., et al., J. Leuk. Biol. 76: 86-94 (2004). 4. Underhill, D.M., et al., Blood 106: 2543-2550 (2005). 5. Lefevre, L., et al., 5(9): e12828 (2010). 6. Fei, M., et al., PNAS USA 108(13): 5360-5365 (2011). 7. Gazi, U., et al., J. Biol. Chem. 286: 7822-7829 (2011). 8. McDonald, J.U., et al., FASEB J. 25(6): 1972-1982 (2011). 9. Dewals, B.G., et al., PLoS Negl. Trop. Dis. 4: e689 (2010). 10. Galès, A., et al., PLoS Pathog. 6: e1000714 (2010). 11. Coates, P.J., et al., Cancer Res. 68: 450-456 (2008). 12. Dioszeghy, V., et al., J. Immunol. 181: 6514-6524 (2008). 13. Hohl, T.M., J. Infect. Dis. 198: 176-185 (2008). 14. Palma, A.S., et al., J. Biol. Chem. 281: 5771-5779 (2006). 15. Sindrilaru, A., et al., J. Clin. Invest. 121: 985-997 (2011). 16. Anandasabapathy, N., et al., J. Exp. Med. 208(8): 1695-1705 (2011).
USBio References
No references available
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