Recovery of bacteria from surfaces cleansed with disinfectants.
Description
D/E Neutralizing Agar is capable of neutralizing a broad spectrum of antiseptic and disinfectant chemicals including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde and glutaraldehyde. It can determine the bactericidal capability of disinfectants and therefore is well suited for environmental sampling.
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light bluish grey color.
Appearance
Blueish-grey, homogeneous, lumpy powder.
Solubility
Purple, opaque, complete.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions
Dissolve 54g/liter in ddH2O with heat. stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Components shown as g/liter |
Yeast Extract | 2.5 |
Dextrose | 10.0 |
Casein Digest Peptone | 5.0 |
Lecithin | 7.0 |
Sodium Thioglycollate | 1.0 |
Sodium Thiosulfate | 6.0 |
Sodium Bisulfite | 2.5 |
Polysorbate 80 | 5.0 |
Bromocresol Purple | 0.02 |
Agar | 15.0 |