Detection of thermophilic, aerobic organisms in foods.
Description
Dextrose Tryptone Agar is recommended for the determination of mesophilic and thermophilic bacteria in sweetening agents used in frozen desserts. Bromcresol purple is used as a pH indicator. When isolating thermophilic bacteria use 55°C as a plating temperature.
Storage and Stability
Store powdered media at 4°C. Opened bottles should be capped tightly. Protect it from moisture and light. Prepared media should be kept at 4°C and used within a short period of time.
Appearance
Light greenish-beige, homogeneous, free flowing powder.
Solubility
Purple, clear to turbid.
Microbiological Analysis
Bacillus coagulans ATCC 7050: Growth yellow colonies Bacillus stearothermophilus ATCC 7953: Growth yellow colonies
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions
1. Dissolve 30grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
| Tryptone | 10.0 |
| Dextrose | 5.0 |
| Bromcresol Purple | 0.04 |
| Agar | 15.0 |
TDS
NCBI
UniProt
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