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D3713-60 1,2-Dioleoyl-3-trimethylammonium-propane, Chloride (DOTAP) CAS: 132172-61-3

Specifications
CAS Number
132172-61-3
Grade
Highly Purified
Molecular Formula
C42H80ClNO4
Molecular Weight
698.55
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
-20°C
DOTAP chloride; N-(1-(2,3-Dioleoyloxy)propyl)-N,N,N-trimethylammonium chloride; N,N,N-Trimethyl-2,3-bis[[(9Z)-1-oxo-9-octadecenyl]oxy]-1-propanaminium

Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10ug DOTAP per ug DNA and approximately 10-20ug DOTAP per ml cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.

Synonyms
DOTAP chloride
CAS No
132172-61-3
Molecular Formula
C42H80ClNO4
Molecular Weight
698.55
Appearance
Yellow to pale beige sticky solid.
Purity
≥96%
Solubility
Chloroform (Slightly), Methanol (Sparingly)
Method for Determining Identity
Proton (CDCl3) NMR and Mass Spectroscopic Analysis
TLC Conditions
SiO2; Dichloromethane: Methanol=8:2; Visualized with UV and KMnO4; Single spot; Rf=0.70
Chloride
As reported
Atmosphere
Inert gas
Storage and Stability
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic. Store under inert atmosphere.
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