*The cohesive ends of the 12 nt cos site of bacteriophage lambda from fragments of 21226 bp and 3530 bp may anneal and form an additional band at 24756 bp. These fragments may be separated by heating at 65°C for 5 minutes and then cooling on ice for 3 minutes.

Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.

Fragment % ng DNA (bp) 21226 43.8 218.8 7421 15.3 76.5 5804 12.0 59.8 5643 11.6 58.2 4878 10.1 50.3 3530 7.3 36.4

Total 100 500

1.One vial is sufficient for ~100 applications. 2. Use 0.1ug of DNA marker (before dilution) per 1mm and an agarose gel lane.

Vortex gently prior to use. 1ul (0.5ug) DNA marker 1ul of 6X Loading Dye Solution 4ul ddH2O Heat for 5 minutes at 65°C. Cool on ice for 3 minutes. Apply the prepared amount (6ul) of the DNA Marker on a 5mm lane of agarose gel. Following electrophoresis separartion on gel, visualize the DNA bands by ethidium bromide staining.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

~0.5mg DNA/ml.

Supplied as a liquid in10mM Tris-HCl, pH 7.6, 1mM EDTA.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.