Supplied with 6x Loading Dye Solution
Lambda DNA was completely digested by both EcoRI and HindIII, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 13 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125.
The cohesive ends of the 12 nt cos site of bacteriophage lambda from fragments of 21226 bp and 3530 bp (indicated*) may anneal and form an additional band at 24756 bp. These fragments may be separated by heating at 65°C for 5min and then cooling on ice for 3min.
Concentration
~0.5mg DNA/ml
Form
Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 1mM EDTA. 6X Loading Dye Solution L3350: Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.
Prepare DNA Marker as follows
1ul (0.5ug) DNA marker 1ul of 6X Loading Dye Solution 4ul ddH2O 1. Vortex gently prior to use 2. Heat 5 min. @ 65°C, cool on ice 3 min. 3. Apply 6ul DNA marker per 5mm of agarose gel lane width. 4. Visualize bands with ethidium bromide staining.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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