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D9500 Drop-out Base With Glucose (DO, Dropout, Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Synthetic minimal media with glucose

Nitrogen, carbon, trace element, and vitamin source for yeast growth used as a media base for yeast transformant selection. Supplement with the appropriate DO Mix for selection and propagation of yeast transformants. Glucose allows for the rapid growth of yeast with short doubling times.

All lots are quality control tested using the appropriate yeast strains and transformants.
• Convenient Powders • Useful for One-Hybrid and Two-Hybrid Systems • Competitively Priced...!
BSE-Free
All raw materials of animal origin are sourced from USDA-designated BSE-Free countries
Dust-Free
Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous
Proprietary blending technology ensures complete mixing of components
Appearance
White, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
pH
5.0 ± 0.5
Directions per Liter
Dissolve 26.7g in ~900ml of ddH2O stirring gently until solubilized. Adjust pH to the desired level. Add water to bring the solution to 1L. Sterilize using a 0.22um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
D-Glucose20.0
YNB (w/o aa w/ ammonium sulfate)6.7
Total26.7g/L
500g makes 18.73 liters
References
1. Kaiser, C., Michaelis, S., Mitchell, A.: Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210 (1994) CSHL Press.
USBio References
No references available
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