Many modifications of Eagle’s Medium have been developed since the original formulation appeared in literature. Among the most widely used of these modifications is Dulbecco’s Modified Eagle’s Medium (DME). DME is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and viatamins, as well as additional supplementary components. The original DME formula contains 1000mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500mg/L glucose has proved to be optimal in cultivating certain cell types.
| Components shown as g/liter |
| Calcium Chloride•2H2O | 0.265 |
| Ferric Nitrate•9H2O | 0.0001 |
| Magnesium Sulfate, Anhy | 0.09767 |
| Potassium Chloride | 0.400 |
| Sodium Chloride | 6.400 |
| Sodium Phosph Mono•H2O | 0.125 |
| L-Arginine•HCl | 0.084 |
| L-Cystine•2HCl | 0.0626 |
| L-Glutamine | Absent |
| Glycine | 0.030 |
| L-Histidine•HCl•H2O | 0.042 |
| L-Isoleucine | 0.105 |
| L-Leucine | 0.105 |
| L-Lysine•HCl | 0.146 |
| L-Methionine | 0.030 |
| L-Phenylalanine | Absent |
| L-Serine | 0.042 |
| L-Threonine | 0.095 |
| L-Tryptophan | 0.016 |
| L-Tyrosine•2Na•2H2O | Absent |
| L-Valine | 0.094 |
| Choline Chloride | 0.004 |
| Folic Acid | 0.004 |
| myo-Inositol | 0.0072 |
| Niacinamide | 0.004 |
| D-Pantothenic Acid•Ca | 0.004 |
| Pyridoxal•HCl | 0.004 |
| Riboflavin | 0.0004 |
| Thiamine•HCl | 0.004 |
| D-Glucose | 4.500 |
| Pyruvic Acid, Sodium | Absent |
| Phenol Red, Sodium | 0.0159 |
| Total: | 12.687 |
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