Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
| Components shown as | mg/liter |
| Calcium Chloride•2H2O | 264.92 |
| Ferric Nitrate | 0.10 |
| Magnesium Sulfate | 97.67 |
| Potassium Chloride | 400 |
| Sodium Chloride | 6400 |
| Sodium Phosphate Monobasic | 108.69 |
| L-Arginine | 84 |
| L-Cystine•2HCI | 62.53 |
| L-Glutamine | 584 |
| Glycine | 30 |
| L-Histidine•HCl | 42 |
| L-Isoleucine | 104.8 |
| L-Leucine | 104.8 |
| L-Lysine•HCI | 146.2 |
| L-Methionine | 30 |
| L-Phenylalanine | 66 |
| L-Serine | 42 |
| L-Threonine | 95.2 |
| L-Tryptophan | 16 |
| L-Tyrosine•Na2•2H2O | 103.79 |
| L-Valine | 93.6 |
| Choline Chloride | 4 |
| Folic Acid | 4 |
| i-Inositol | 7 |
| Nicotinamide | 4 |
| D-Pantothenic Acid•Ca | 4 |
| Pyridoxal•HCI | 4 |
| Riboflavin | 0.4 |
| Thiamine•HCI | 4 |
| D-Glucose | 4500 |
| HEPES | 5958 |
| Phenol Red | 15.34 |
| Sodium Pyruvate | 110 |
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