Dulbecco’s MEM

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.

Appearance: Orange to light orange, homogeneous, free flowing powder Solubility: Yellow, clear, complete pH: As reported Endotoxin: ≤10EU/mg

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 15.554g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as

mg/liter

Inorganic Salts

Calcium Chloride•2H20	154.52
Cupric Sulfate•5H2O	Absent
Ferric Nitrate•9H2O	0.05
Ferrous Sulfate•7H2O	Absent
Magnesium Chloride	28.57
Magnesium Sulfate	Absent
Potassium Chloride	311.83
Sodium Chloride	6999.5
Sodium Phosphate Dibasic	71.06
Sodium Phosphate Monobasic	54.35
Zinc Sulfate•7H2O	Absent

Amino Acids

L-Alanine	4.455
L-Arginine	147.35
L-Asparagine•H2O	8.53
L-Aspartic Acid	6.655
L-Cysteine•HCI•H2O	17.65
L-Cystine•2HCI	31.29
L-Glutamic Acid	7.355
L-Glutamine	365.1
Glycine	18.755
L-Histidine HCl•H2O	31.48
L-Isoleucine	54.368
L-Leucine	58.96
L-Lysine•HCI	91.37
L-Methionine	17.238
L-Phenylalanine	35.478
L-Proline	17.265
L-Serine	26.255
L-Threonine	53.555
L-Tryptophan	9.02
L-Tyrosine•2Na•2H20	55.813
L-Valine	52.655

Vitamins

Biotin	0.00367
Choline Chloride	8.98
Vitamin B12	0.679
Folic Acid	2.66
i-Inositol	12.600
Nicotinamide	2.018
D-Pantothenic Acid•Ca	2.12
Pyridoxal HCI	2
Pyridoxine HCI	0.031
Riboflavin	0.219
Thiamine HCI	2.17

Other

Thymidine	0.365
D-Glucose	3150
HEPES Free Acid	3574
Hypoxanthine, Sodium	2.1
Linoleic Acid	0.042
Lipoic Acid	0.105
Phenol Red	8.305
Putrescine•2HCl	0.081
Sodium Pyruvate	55.05

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D9811-10A Dulbecco’s MEM (DMEM) Ham’s F-12 w/HEPES w/o CuSO4, FeSO4, MgSO4, ZnSO4 (Powder)

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