Each antibody in the Phospho-Erk1/2 Pathway Antibody Sampler Kit recognizes only the phosphorylated form of its specific target.

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, cell differentiation, cell movement and cell death. MAPK signaling cascades are organized hierarchically into three-tiered modules. MAPKs are phosphorylated and activated by MAPK kinases (MAPKKs), which in turn are phosphorylated and activated by MAPKK-kinases (MAPKKKs). The MAPKKKs are in turn activated by interaction with a family of small GTPases and/or other protein kinases connecting the MAPK module to the cell surface receptor or external stimuli. The Phospho-Erk1/2 Pathway Sampler BioAssay™ Kit provides a fast and economical means to evaluate the activation status of the entire Erk1/2 pathway. The kit includes enough primary and secondary antibodies to perform two Western mini-blot experiments, as well as a specific inhibitor of MEK1/2 (U0126).

Purified by protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Each antibody in the Phospho-Erk1/2 Pathway Sampler Kit recognizes only the phosphorylated form of its specific target.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.