ETV4, CT

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

P43268

Isotype

IgG

Grade

Purified

Applications

E FC IHC WB

Crossreactivity

Hu Mo

Accession #

NP_001977

Shipping Temp

Blue Ice

Storage Temp

-20°C

ETV4 is a transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.

Applications

Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications not tested.

Recommended Dilutions

ELISA: 1:1000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50- 1:100 Indirect Flow Cytometry: 1:10- 1:50 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa426-455 from the C-terminal region of human ETV4.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by ammonium sulfate precipitation.

Specificity

Recognizes human ETV4. Species Crossreactivity: mouse.

References

1. Wei,Y., J. Biochem. 144(4), 539-546 (2008).|2. Park, Sung-Won, et al. Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation. 2014. Biol. Phar. Bull. 37(4):659-665.

USBio References

1. Sung-Won Park, Hyun-Jin Do, Woo Tae Ha, Mi-Hee Han,a Hyuk Song, Sang-Jun Uhm, Hak-Jae Chung, and Jae-Hwan Kim. Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation. 2014. Biol. Phar. Bull. 37(4):659-665. https://www.jstage.jst.go.jp/article/bpb/37/4/37_b13-00985/_pdf

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E8851-16 Rabbit Anti-ETV4, CT (ETS Translocation Variant 4, Polyomavirus Enhancer Activator 3 Homolog, Protein PEA3, Adenovirus E1A Enhancer-binding Protein, E1A-F, E1AF)

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