EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the center region of human EIF4B (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human EIF4B.