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F0024-43A Goat Anti-FBXO43 (F-box Only Protein 43, Endogenous Meiotic Inhibitor 2, EMI2, FBX43)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IF WB
Crossreactivity
Bo Ca Hu Mo Po Rt
Accession #
NP_001025031.2
Gene ID
286151, 78803, 315034
Shipping Temp
Blue Ice
Storage Temp
-20°C

Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-PGSAQSKRNLKRL, from rat FBXO43, at C-terminal (NP_001025031.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat FBXO43. Species sequence homology: Bovine, canine, human, mouse and porcine.
References
1. Perrard MH, Chassaing E, Montillet G, Sabido O, Durand P. Cytostatic Factor Proteins Are Present in Male Meiotic Cells and β-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes. PLoS One. 2009 Oct 5;4(10):e7237.
USBio References
No references available
Conjugates
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