Fluid Thioglycollate Medium with 0.5% Beef Extract is recommended by Animal and Plant Health Inspection Service, USDA, in the detection of viable bacteria in live vaccines. Various workers described the use of sulfhydryl compounds and low concentrations of agar, alone or in combination, to culture anaerobic organisms in a fluid broth under aerobic conditions. Other workers described the use of sodium thioglycollate in these broth media for the neutralization of mercurial preservatives. Sodium thioglycollate aIso lowers the oxidation-reduction potential of the media. Resazurin indicates the status of oxidation or aerobiosis. Dextrose is added to enhance growth.
Appearance
Light beige, homogeneous, free flowing powder.
Solubility
Yellow, hazy to clear, complete with upper 10% of solution light to medium red upon standing.
Microbiological Analysis
Bacillus subtilis ATCC 9372: Growth Bacteroides vulgatus ATCC 8482: Growth Candida albicans ATCC 10231: Growth Clostridium sporogenes ATCC 11437: Growth Micrococcus luteus ATCC 9341: Growth
Recommended by Agriculture Research Service CFR Amendment 70-253, 35FR16038, 10, 13, 70,
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige.
Directions
1. Dissolve 34.7grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Note: After storage of the medium, if 30% of the medium demonstrates oxidation (color change of indicator), reheat the medium once in boiling water or steam bath.
Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
TDS
NCBI
UniProt
Tech Support
Customer Service
