Suitable for use in Direct ELISA, Western blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5-1ug/ml. Western blot: 0.1-0.2ug/ml The detection limit for rmFrizzled-4 is approximately 2ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15ug/ml in fixed cells or tissues. Optimal dilutions to be determined by the researcher
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a liquid in PBS, pH 7.2, before the addition of 40% glycerol.
Purity
Purified by immunoaffinity chromatography. Endotoxin: <0.1Eu/ug
Specificity
Recognizes mouse Frizzled-4 extracellular Cysteine-Rich Domain (CRD). ~5% crossreactivity with rmFrizzled-8 and less than 2% crossreactivity with rmFrizzled-3 and rmFrizzled-7.
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