Mycobiotic Agar
Selective medium with nearly neutral pH, used to isolate pathogenic fungi.
Description
Fungisel agar contains chloramphenicol and cycloheximide. These antibiotics selectively inhibit saprophytic fungi and bacteria while allowing pathogenic fungi to grow. Various workers demonstrated the use of these antibiotics in Sabouraud Dextrose Agar and other media for the isolation of pathogenic fungi from specimens with mixed flora.
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 8.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
Appearance
Light beige, homogeneous, free flowing powder.
Solubility
Light to medium yellow, hazy to clear, complete.
Microbiological Analysis
Aspergillus niger ATCC 16404: Inhibited Candida albicans ATCC 10231: Growth Microsporum audouinii ATCC 42558: Growth Staphylococcus epidermidis ATCC 12228: Inhibited
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions
1. Dissolve 36grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
2. Distribute and autoclave for at 121.0°C for 15 minutes. Cool to room temperature, use immediately. Do not remelt more than once. Do not hold medium at 45-50°C for more than 30 minutes. DO NOT OVERHEAT.
Storage
Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be stored at 4°C and used within a short period of time.
Components are shown as g/Liter
Papaic Digest of Soybeans | 10.0 |
Dextrose | 10.0 |
Chloramphenicol | 0.05 |
Cycloheximide | 0.4 |
Agar | 15.5 |