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G1015-71 Rabbit Anti-GABA Transporter 2 (GAT-2 (Gamma aminobutyrIc acid) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

GABA is a major inhibitory neurotransmitter and the GABAergic transmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters. It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties. Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1). There is ~50% homology between various GABA transporter subtypes. GABA transporters are predicted to contain 12 potential transmembrane domains. The NH2 and COOHtermini are predicted to be intracellular. Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity). GAT1and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues. It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000. ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies. Optimal dilutions to be determined by the researcher.
Control Peptide
G1015-72: GABA Transporter 2, Rat Control Peptide (GAT-2, Rat (Gamma aminobutyrIc acid)
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 15aa synthetic peptide near the C-terminus of rat GAT2 (KLH).
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Purity
Serum
Specificity
Recognizes rat GABA Transporter 2. No significant homology to any other known eukaryotic proteins. Species sequence homology: mouse,100%.
References
1. Liu, Q. R. et al (1993) J. Biol. Chem. 268:2106-2112; Borden, L. A. et al (1992) J. Biol. Chem. 267:21098-21104. 2. Ikegaki, N. et al (1994) Mol. Brain Res. 26:37-46
USBio References
No references available
Conjugates
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