GABA is a major inhibitory neurotransmitter and the GABAergic transmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters. It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties. Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1). There is ~50% homology between various GABA transporter subtypes. GABA transporters are predicted to contain 12 potential transmembrane domains. The NH2 and COOH-termini are predicted to be intracellular. Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity). GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues. It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions. Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies. Western Blot: 1-10ug/ml using chemiluminescence technique. The use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific band. Immunohistochemistry: 2-10ug/ml in paraformaldehyde fixed sections of tissue. Optimal dilutions to be determined by the researcher.

G1015-72: GABA Transporter 2, Rat Control Peptide

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

A synthetic peptide corresponding to15aa near the C terminus of rat GABA Transporter 2, conjugated to KLH.

Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.

Purified by immunoaffinity chromatography.

Recognizes rat GABA Transporter 2. No significant homology to any other known eukaryotic protein. Species Sequence Homology: mouse