The Human GFAP BioAssay™ ELISA is a biotin-labeled-antibody-based sandwich enzyme immunoassay for the quantitative measurement of human GFAP in serum, CSF, plasma and tissue culture medium. It is intended for in vitro and research use only.

• The total assay time is less than five hours. • The kit measures total serum, CSF, or plasma GFAP. • Quality Controls are human serum based. No animal sera are used. • Standard is purified native protein based

Glial Fibrillary Acidic Protein (GFAP), as a member of the cytoskeletal protein family, is the principal 8-9nm intermediate filament in mature astrocytes of the central nervous system (CNS). GFAP is a monomeric molecule with a molecular mass between 40 and 53kD and an isoelectric point between 5.7 and 5.8. GFAP is a highly brain-specific protein that is not found outside the CNS. Findings have shown that while GFAP is released into the blood very soon after traumatic brain injury (TBI) based on its severity, GFAP is not released after even multiple traumae in the absence of brain injury. In the CNS, following injury, either as a result of trauma, disease, genetic disorders, or chemical insult, astrocytes become reactive and respond in a typical manner, termed astrogliosis. Astrogliosis is characterized by the rapid synthesis of GFAP. GFAP normally increases with age and there is a wide variation in the collection and processing of human brain tissue. Thanks to its high brain specificity and early release from the CNS after TBI, GFAP might be a suitable marker for early diagnostics.

In Human GFAP ELISA calibrators or samples are incubated with a rabbit polyclonal anti-human GFAP antibody coated in microtiter wells. After two hours of incubation and a washing, biotin-labeled monoclonal anti-human GFAP antibody is added and incubated with captured GFAP. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour of incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution. The absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of GFAP. A standard curve is constructed by plotting absorbance values versus GFAP concentrations of calibrators, and concentrations of unknown samples are determined using this standard curve.

G2032-29A: Microtiter Strips, coated with capture polyclonal antibody, sealed, 1x96 wells *G2032-29B: Human GFAP Master Calibrator, 1x1 vial *G2032-29C: Quality Control: High, 6.3-11.8ng/ml, 1x1 vial *G2032-29D: Quality Control: Low, 1.4-2.6ng/ml, 1x1 vial G2032-29E: Pab (Biotin), 1x13ml G2032-29F: Streptavidin (HRP), 1x13ml G2032-29G: Standard Diluent, 1x9ml G2032-29H: Dilution Buffer, 1x13ml G2032-29J: Wash Solution, 10X, 1x100ml G2032-29K: TMB Substrate Solution, 1x13ml G2032-29L: Stop Solution, H2SO4, 1x13ml

Store at 4°C. Stable for 6 months after receipt at 4°C.*Note: Store G2032-29B, G2032-29C and G2032-29D at 4°C as a lyophilized powder. Upon reconstitution store at -20°C. Stable for 3 months at -20ºC.