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G2033-72 Ghrelin, Human, BioAssay™ ELISA Kit

Specifications
References
Brand
BioAssay™
Kit Type
ELISA
Tests
96
Sample Volume
50ul/well
Sensitivity
0.13ng/ml
Detection Method
Colorimetric/Fluorescent
Detection Range
0-100ng/ml
Sample Matrix
Plasma, serum, culture media, tissue homogenate, CSF and urine
EU Commodity Code
38220000
UN DOT Shipping
UN1789 PGIII
Shipping Temp
Blue Ice
Storage Temp
4°C

Peptide Sequence
Gly-Ser-Ser(n-Octanoyl)-Phe-Leu-Ser-Pro-Glu-His-Gln-Arg-Val-Gln-Gln-Arg-Lys-Glu-Ser-Lys-Lys-Pro-Pro-Ala-Lys-Leu-Gln-Pro-Arg
Sensitivity
Minimum Detectable Concentration: As reported
Precision
Intra-assay error: <10% Inter-assay error: <15%
Range
0-100ng/ml
Linear Range
As reported
Plasma Extraction
Extraction is strongly recommended but not required.
Specificity
Peptide %Crossreactivity Ghrelin (Human, Rat, Mouse) 100 Ghrelin 1-27 (Human) (O-n-Octanoyl-Ser3) 100 Ghrelin 1-27 (Human) (O-n-Decanoyl-Ser3) 100 Ghrelin (Human) (Des-Octanoyl-Ser3) 100 Ghrelin (Rat) (Des-Octanoyl-Ser3) 100 Ghrelin (CT) 100 Ghrelin, Gln28 (Human) 100 Ghrelin (Canine) 100 Ghrelin (Monkey) 85 Ghrelin (Porcine) 0 Secretin (Human) 0 VIP (Human, rat, mouse, porcine,sheep) 0 Prolactin-Releasing Peptide-31 (Rat) 0 Prolactin-Releasing Peptide-31 (Human) 0 Galanin (Rat) 0 Galanin (Human) 0 GH-RF (Rat) 0 GH-RF (Human) 0 NPY (Human, Rat, Mouse) 0 Orexin A (Human, Mouse, Rat, Bovine) 0 Orexin B (Human)
Kit Components
G2033-72A: Assay Buffer (20x), 1x50ml G2033-72B: Microtiter Plate, 1x96 well G2033-72C: Ghrelin Pab, 1x1vial G2033-72D: Standard, 1x1 vial (1ug) G2033-72E: Peptide (Biotin), 1x1vial G2033-72F: Streptavidin (HRP), 1x30ul G2033-72G: Substrate, 1x12ml G2033-72H: Stop Solution, 2N HCI, 1x15ml G2033-72J: Positive Control, 2x200ul
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
It is recommended that the solutions be used on the same day of rehydration. Blood collection protocol and extraction procedure for plasma is provided for your information. (Materials for extraction not included).
Source
Human
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Ahluwalia, A. et al., (2009) J Physiol Pharmacol. 60: 29-34. 2. Li, A. et al., (2007) BBRC 353:238-243. 1. Porstmann, T. and Kiessig, S.T., Journal of Immunological Methods, 150: 5-21 (1992). 2. Avrameas, S., Journal of Immunological Methods, 150: 23-32 (1992). 3. Shuto, et al., J. Clin. Invest. 109(11): 1429-1436 (2002).
USBio References
US Biological application references: 1. Ahluwalia, A. et al., (2009) J Physiol Pharmacol. 60: 29-34. 2. Li, A. et al., (2007) BBRC 353:238-243.
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