Glucoamylase is an enzyme preparation which decomposes starch into glucose by tearing-off glucose units from the non-reduced end of the polysaccharide chain. It is derived by submerged fermentation of a specially selected producer strain of Asp. niger.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:5000-1:75,000; 1ug of Glucoamylase was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT. Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Glucoamylase (Rhizopus)
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 100ul sterile dH2O. Labeled with Biotin.
Purity
Purified by delipidation, salt fractionation and ion exchange chromatography.
Specificity
Recognizes Glucoamylase. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Glucoamylase (Rhizopus).
TDS
NCBI
UniProt
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