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G4010-03 Glutamate Pyruvate Transaminase, Recombinant, Human, aa1-496, His-Tag (Alanine Aminotransferase 1, ALT1, Glutamate Pyruvate Transaminase 1, GPT 1, Glutamic-alanine Transaminase 1, Glutamic-pyruvic Transaminase 1, AAT1, GPT1, GPT) CAS:

Specifications
References
Grade
Highly Purified
Accession Number
NP_005300
Molecular Weight
56.8
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

GPT, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and aa. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings.

Source
Recombinant corresponding to aa1-496 from human GPT, fused to His-tag at N-terminal expressed in E.coli.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
Molecular Weight
~56.8kD (MALDI-TOF)
Enzyme Activity
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~90% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 20% glycerol, 2mM DTT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Sohocki M.M. et al. (1997) Genomics 40: 247-252. 2. Matthews C.C. et al. (2003) Brain Res. 978: 59-64.
USBio References
No references available
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