Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000. Antiserum yields positive result with 0.1ug of purified rat GST Ya, or recombinant human A1-1, and a 1:5000 dilution of secondary antibody. Optimal dilutions to be determined by researcher.
Recommended Secondary Antibody
I1903-28A IgG, X-Adsorbed (AP) Dk x Gt I1903-30B IgG, H&L, X-Adsorbed (AP) Rb x Gt
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Affinity purified rat liver GST Ya.
Form
Supplied as a liquid (neat serum).
Purity
Serum
Specificity
Recognizes rat Glutathione S Transferase Ya. Does not crossreact with mu or pi class GST. Species Crossreactivity: human
References
1. Holmes, W., et.al., Br. J. Cancer, 62 : 209-212 (1990)