GAPDH, NT

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

P04406

Isotype

IgG

Grade

Purified

Applications

E IHC WB

Crossreactivity

Accession #

NP_002037

Shipping Temp

Blue Ice

Storage Temp

-20°C

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Applications

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide selected from the N-terminal region of human GAPDH (KLH).

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by ammonium sulfate precipitation.

Specificity

Recognizes human GAPDH.

References

1.Azam,S., J. Biol. Chem. 283(45), 30632-30641 (2008). 2.Lu,J., Biosci. Biotechnol. Biochem. 72(9), 2432-2435 (2008). 3.Zhou,Y., Mol. Cancer Res. 6(8), 1375-1384 (2008).