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G8179-11 Mouse Anti-Glycoprotein 340 (gp340)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
6F122
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glycoprotein 340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp340 has been shown to bind SP-D in a calcium dependent manner through a protein-protein interaction. Like SP-D, gp340 is mainly found in lung tissue and immunohistochemistry revealed high expression in alveolar macrophages (3). An identical protein is present in saliva (salivary agglutinin) and both forms are encoded by the gene DMBT1 (6).

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Native gp340 purified from bronchioaveolar lavage from proteinosis patients.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Glycoprotein 340 (gp340). In Western Blot after SDS-PAGE, reacts strongly with gp340 in non-reduced forms.
References
1. Ligtenberg, T.J., et al., Biochem J. 359: 243-248 (2001). 2. Bikker, F.J., et al., J. Dent. Res. 81: 134-139 (2002). 3. Holmskov, U., et al., Proc. Natl. Acad. Sci. USA 96: 10,794-10,799 (1999). 4. Bisgaard, H.C., et al., Am J. Pathol. 161: 1187-1198 (2002). 5. Prakobphol, A., et al., J. Biol. Chem. 275: 39,860-39,866 (2002). 6. Bikker, F.J., et al., J. Biol. Chem. 277: 32,109-32,115 (2002). 7. Kang, W., et al., Clin. Exp. Immunol. 130: 449-458 (2002).
USBio References
No references available
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