Appearance
White, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
Directions per Liter
Dissolve 44.4g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Components shown as g/liter
Inorganic Salts
| Calcium Chloride | 1.0 |
| Magnesium Chloride | 1.068189 |
| Magnesium Sulfate | 1.357858 |
| Potassium Chloride | 2.24 |
| Sodium Phosphate Mono | 0.876923 |
Amino Acids
| L-Alanine | 0.225 |
| ß-Alanine | 0.2 |
| L-Arginine•HCl | 0.7 |
| L-Asparagine | 0.35 |
| L-Aspartic Acid | 0.35 |
| L-Cystine•2HCl | 0.025 |
| L-Glutamic Acid | 0.6 |
| L-Glutamine | 0.6 |
| Glycine | 0.65 |
| L-Histidine | 2.5 |
| L-Isoleucine | 0.05 |
| L-Lysine•HCl | 0.625 |
| L-Phenylalanine | 0.15 |
| L-Proline | 0.35 |
| DL-Serine | 1.1 |
| L-Threonine | 0.175 |
| L-Tryptophan | 0.1 |
| L-Tyrosine•2Na | 0.07202 |
| L-Valine | 0.1 |
Vitamins
| p-Aminobenzoic Acid | 0.00002 |
| D-Biotin | 0.00001 |
| Choline Chloride | 0.0002 |
| Folic Acid | 0.00002 |
| Myo-Inositol | 0.00002 |
| Niacin | 0.00002 |
| D-Pantothenic Acid•Ca | 0.00002 |
| Pyridoxine•HCl | 0.00002 |
| Riboflavin | 0.00002 |
| Thiamine | 0.00002 |
Other
| D(-)Fructose | 0.4 |
| Fumaric Acid | 0.055 |
| D(+)-Glucose | 0.7 |
| a-Ketoglutaric Acid | 0.37 |
| L(-)-Malic Acid | 0.67 |
| Succinic Acid | 0.06 |
| Sucrose | 26.68 |
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