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G8959-13 Goat Anti-GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_004801.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mona, Grf40, RP3-370M22.1, growth factor receptor-bound protein 2-related adaptor protein 2, hematopoietic cell-associated adaptor protein GRPL, GRB2-related protein with insert domain, growth factor receptor-binding protein, SH3-SH2-SH3 adaptor molecule, GRB-2-like protein, GRB2-related adaptor protein 2, GRAP-2, GRBLG, GRB2L, Mona, GrbX, GRPL, GADS, P38, GRID, GRAP2

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.

Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:32,000 Western Blot: 1-3ug/ml detects ~38kD band observed lysates of cell line Jurkat (calculated MW of 37.9kD according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kD is observed. This band is not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-GLFPANYVAPMTR, from the C Terminus of human GRAP2 (NP_004801.1).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human GRAP2. Species sequence homology: mouse, porcine.
References
1.Qiu M, Hua S, Agrawal M, Li G, Cai J, Chan E, Zhou H, Luo Y, Liu M.Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1.Biochem Biophys Res Commun. 1998 Dec 18;253(2):443-7.
USBio References
No references available
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